RpoB Gene Mutation Characteristics of Rifampicin-resistant Tuberculosis in Anqing, China

Background: Rifampicin resistant tuberculosis is a serious problem faced by control in China, and rapid detection of rifampicin resistance urgently needed. Objectives: This study aimed to describe the molecular characteristics frequency RNA polymerase β subunit (rpoB) gene mutations rifampicin-resistant (RR-TB) Anqing area. Methods: The rpoB fragment was amplified chain reaction (PCR), all isolates were sequenced for gene. obtained comparing sequencing results with MUBII database. In addition, logistic regression used analyze relationship between (RIF) resistance. Results: There 152 males 42 females this study, mean age 56.60 ± 17.91 years. Mutations risk factor (β = 5.271, P < 0.001 OR 195.192). Among 19 RR-TB strains, 16 (84.21%) had ropB gene, three (1.71%) 175 rifampicin-sensitive strains mutated. mutation sites five (31.58%) at codon 526 531. However, there two 513 533 (15.79%), (10.53%) double mutations. Conclusions: area are complex, can be as an indicator screen drug RIF.